human whole-blood cd66b positive selection kit  (STEMCELL Technologies Inc)

Journal: Immunity

Article Title: DNA methylation signatures reveal that distinct combinations of transcription factors specify human immune cell epigenetic identity

doi: 10.1016/j.immuni.2021.10.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Granulocytes were positively selected from whole blood using EasySepTM Human Whole Blood CD66b Positive Selection Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

Techniques: Saline, Selection, DNA Methylation Assay, Sequencing, Methylation, Software, DNA Extraction

Journal: Immunity

Article Title: DNA methylation signatures reveal that distinct combinations of transcription factors specify human immune cell epigenetic identity

doi: 10.1016/j.immuni.2021.10.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Granulocytes were positively selected from whole blood using EasySepTM Human Whole Blood CD66b Positive Selection Kit (StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

Techniques: Saline, Selection, DNA Methylation Assay, Sequencing, Methylation, Software, DNA Extraction

Journal: Journal of Inflammation Research

Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

doi: 10.2147/JIR.S347777

Figure Lengend Snippet: CD66b+ neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: PANs and TANs were isolated with EasySep TM human whole blood CD66b positive selection kit (18,682, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

Techniques: Immunohistochemistry

Journal: Journal of Inflammation Research

Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

doi: 10.2147/JIR.S347777

Figure Lengend Snippet: Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: PANs and TANs were isolated with EasySep TM human whole blood CD66b positive selection kit (18,682, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

Techniques: Expressing, Flow Cytometry